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Table 4 Specific primers targeting the Internal Transcribed Spacer Region-1 (ITS-1) of different Eimeria species in chickens

From: Epidemiological investigation and drug resistance of Eimeria species in Korean chicken farms

Species

Primer sequences 5’-3’

Expected amplicon size (bp)

Annealing Temperature (°C)

References

ITS-1

F

AAGTTGCGTAAATAGAGCCCTC

variable

56

[62]

R

AGACATCCATTGCTGAAAG

E. acervulina

F

GGGCTTGGATGATGTTTGCTG

145

65

[61]

R

GCAATGATGCTTGCACAGTCAGG

E. brunetti 1

F

CTGGGGCTGCAGCGACAGGG

183

58

[62]

R

ATCGATGGCCCCATCCCGCAT

E. brunetti 2

F

GATCAGTTTGAGCAAACCTTGC

311

65

[61]

R

TGGTCTTCCGTACGTCGGAT

E. maxima 1

F

GTGGGACTGTGGTGATGGGG

205

65

 

R

ACCAGCATGCGCTCACAACCC

E. maxima 2

F

TTGTGGGGCATATTGTTGTGA

162

60

 

R

CWCACCACTCACAATGAGGCAC

E. mitis

F

GTTTATTTCCTGTCGTCGTCTCGC

330

65

 

R

GTATGCAAGAGAGAATCGGGATTCC

E. necatrix

F

AGTATGGGCGTGAGCATGGAG

160

58

 

R

GATCAGTCTCATCATAATTCTCGCG

E. praecox

F

CATCGGAATGGCTTTTTGAAAGCG

215

65

 

R

GCATGCGCTAACAACTCCCCTT

E. tenella

F

AATTTAGTCCATCGCAACCCTTG

278

65

 

R

CGAGCGCTCTGCATACGACA