Development and evaluation of a bead-based Multiplexed Fluorescent ImmunoAssay (MFIA) for detection of antibodies to Salmonella enterica serogroup B and C1 in pigs

Table 4 Proficiency test results for samples tested from 2015 to 2020 in MFIA and Mix ELISA

				Number of proficiency test samples correctly identified as negative or positive^a
*Salmonella enterica* serovar	Serogroup	O-antigen	# samples tested	MFIA serogroup B	MFIA serogroup C₁	Mix ELISA Serogroup B + C₁
Negative	-	-	11	11	11	11
Brandenburg	B	4,[5],12	8	8	0	4
Typhimurium	B	1,4,[5],12	14	14	0	14
Livingstone	C₁	6,7,14	6	0	6	0
Goldcoast^b	C₂	6,8	3	0	0	0
Panama^c	D₁	1,9,12	6	3	0	3

^aSample ID is unknown to proficiency test participants, so samples may reoccur from year to year
^bThe O-antigens used in MFIA and mix ELISA are not 6,8
^cSince the D₁ O-antigen is not included in the test, the reactants observed are due to cross-reaction with the serogroup B O-antigen

ISSN: 1746-6148