Table 1 Primer sequences used for qPCR analysis of in vitro and in vivo osteogenic gene expressions
From: Enhancement of critical-sized bone defect regeneration using UiO-66 nanomaterial in rabbit femurs
Primer | Primer Sequences | |
|---|---|---|
Forward | Reverse | |
Human Collagen-I | 5’- CAG CCG CTT CAC CTA CAG C -3’ | 5’- TTT TGT ATT CAA TCA CTG TCT TGC C -3’ |
Human Osteocalcin | 5’- ACA CTC CTC GCC CTA TTG -3’ | 5’- GAT GTG GTC AGC CAA CTC -3’ |
Human Osteopontin | 5’- CTC AGG CCA GTT GCA GCC -3’ | 5’- CAA AAG CAA ATC ACT GCA ATT CTC -3’ |
Human GAPDH | 5’-ACA GTC AGC CGC ATC TTC TT-3’ | 5’-GAC AAG CTT CCC GTT CTC AG-3’ |
Rabbit Osteopontin | 5’-GCTCGATGGCTAGCTTGTCT-3’ | 5’-ACAATATAAGCGCGAGGCCA-3’ |
Rabbit Osteocalcin | 5’-GTTCCCTTCCTCCTTGATTT-3’ | 5’-TCTACCAGTTGCAGCCTGAC-3’ |
Rabbit Beta-actin | 5-’CAGGAAGGAGGGCTGGAACA-3’ | 5’-ATCGTGCGGGACATCAAGGA-3’ |