Table 5 Primers and probes used for PCR-based assays for detection of the germline APC variant
Sequence | Product size (bp) | |||
|---|---|---|---|---|
Primers used for PCR-direct sequencing | 385 | |||
Primers | sense | 5’- AGTCCCACCTTCAAAAATCC | ||
antisense | 5’- AACTAAAAATGCAATTATCTTGAATG | -3’ | ||
Primers used for PCR-RFLP and PCR-direct sequencing of FFPE samples | -3’ | 156 | ||
Primers | sense | 5’- TCTTTTGGCATTGTGTAAACTTG | ||
antisense | 5’- CTTACATTTTCAGTTAAAGGGAGACT | -3’ | ||
Primers used and probes for TaqMan realtime PCR of genomic bank samples | -3’ | 78 | ||
Primers | sense | 5’- TTTAGTGAGATTCTGAAGTTGAGCATAATA | -3’ | |
Antisense | 5’- TCATTACTTCTTGCTGATCTTGACAA wild-type allele | -3’ | ||
5’- (VIC)- CAATCTTTTTCCTTTTC-(MGB) | -3’ | |||
Probes mutant allele | 5’- (FAM)-CAATCTTAATCCTTTTC-(MGB) | -3’ | ||