Table 4 Real-time PCR primer sequences
From: Impact of bisphenol-A on the spliceosome and meiosis of sperm in the testis of adolescent mice
Gene ID | Sequence(5'-3') | GC % | Tm℃ | Length (bp) | Amplification efficiency(%) | R2 | |
|---|---|---|---|---|---|---|---|
Snrpc | F | AACGGCTTGTGGAGCATCA | 52.6 | 62.8 | 177 | 101.2 | 0.994 |
R | TGTCTTGTCAATCAGGCTCTGG | 50 | 62.7 | ||||
Hnrnpu | F | TGCCCAACAGAGGGAACTATAACC | 50 | 64.5 | 142 | 97.3 | 0.993 |
R | CCTTGGTGATAATGCTGACTCCA | 47.8 | 63.5 | ||||
β-actin | F | TCCTTCCTGGGCATGGAGT | 57.9 | 63 | 104 | 96.1 | 0.998 |
R | AGCACTGTGTTGGCGTACAG | 55 | 60 | ||||
GAPDH | F | TGCTGGTGCTGAGAGTATGTGGTG | 54.2 | 64.7 | 293 | 104.1 | 0.995 |
R | TCTTCTGGGTGGCAGTGATGG | 57.1 | 62.1 |