Isolation and molecular confirmation of Brucella suis biovar 2 from slaughtered pigs: an unanticipated biovar from domestic pigs in Egypt

Table 1 Diagnostic characteristics of Brucella suis isolates from slaughtered pigs in this study

ID	Genus specific PCR	MALDI-TOF Analysis	AMOS-PCR	Suis-ladder multiplex PCR	H₂S production	Agglutination with Monospecific antisera
ID	Genus specific PCR	MALDI-TOF Analysis	AMOS-PCR	Suis-ladder multiplex PCR	H₂S production	A	M
1	+	Brucella spp	-	B. suis bv 2	+	+	-
2	+	Brucella spp	-	B. suis bv 2	+	+	-
3	+	Brucella spp	-	B. suis bv 2	+	+	-
4	+	Brucella spp	-	B. suis bv 2	+	+	-
5	+	Brucella spp	-	B. suis bv 2	+	+	-
6	+	Brucella spp	-	B. suis bv 2	+	+	-
7	+	Brucella spp	-	B. suis bv 2	+	+	-
8	+	Brucella spp	-	B. suis bv 2	+	+	-
9	+	Brucella spp	-	B. suis bv 2	+	+	-
10	+	Brucella spp	-	B. suis bv 2	+	+	-
11	+	Brucella spp	-	B. suis bv 2	+	+	-
12	+	Brucella spp	-	B. suis bv 2	+	+	-
13	+	Brucella spp	-	B. suis bv 2	+	+	-
14^R	+	Brucella spp	-	B. suis bv 2	-	-	-

All B. suis isolates were positive for the Urease test (rapid 1 min) and negative for growth on Thionine agar (1/25,000); ^R: Rough strain; + : Positive; -: Negative

ISSN: 1746-6148