Fig. 3

Depletion of Caspase-1 can reduce liver damage and inflammation caused by hepatic IRI. A, B and C The hepatocellular function in serum samples was evaluated by detecting ALT, AST, and LDH levels (n = 3). D Representative images of hematoxylin and eosin (HE) staining (scale bar, 100 μm). E and F IL-1β and IL-18 were measured with ELISA in liver samples of WT and Caspase1^−/− mice with Hepatic IRI and sham intervention (n = 6). G Examination of the proteolytic cleavage of Caspase-1 and GSDMD in mice liver specimens with Hepatic IRI or sham, using immunoblotting analysis. GSDMD-FL, full-length GSDMD; GSDMD-N, the N-terminal cleavage product of GSDMD (n = 6). All data are shown as the mean ± SEM. ****P < 0.0001, ***P < 0.001, **P < 0.01 and *P < 0.05