Fig. 2

Caspase-1-GSDMD-induced pyroptosis occurs in the mice hepatic IRI model. A-E Relative expression levels of inflammasome-associated genes were determined using qRT-PCR in mice liver specimens (n = 6). F Representative images of caspase-1 staining (scale bar, 100 μm), the area of Caspase-1-positive cells in the livers were also determined (n = 6). G Caspase-1 activities were measured in mice hepatic IRI liver tissues (n = 6). H Examination of the proteolytic cleavage of Caspase-1 and GSDMD in mice liver specimens with Hepatic IRI or sham intervention, using immunoblotting analysis. GSDMD-FL, full-length GSDMD; GSDMD-N, the N-terminal cleavage product of GSDMD (n = 6). All data are shown as the mean ± SEM. ****P < 0.0001, ***P < 0.001