Table 3 Real-time quantitative RT-PCR probes and primers
From: Influence of fermented feed additive on gut morphology, immune status, and microbiota in broilers
Gene | Sequence type-Probe/primer sequence | Accession number |
|---|---|---|
IL-1β | forward 5′--TTCATTACCGTCCCGTTG-3′ | (NM_204524.1) |
reverse 5′--GCTTTTATTTCTCCAGTCACA-3′ | ||
IL-6 | forward 5′--AAATCCCTCCTCGCCAATCT-3 reverse 5′--CCCTCACGGTCTTCTCCATAAA-3 | (NM_204628.1) |
IL-10 | forward 5′--CGCTGTCACCGCTTCTTCA-3 reverse 5′--TCCCGTTCTCATCCATCTTCTC-3 | (NM_001004414.2) |
IL-4 | forward 5′--GTGCCCACGCTGTGCTTAC −3 reverse 5′--AGGAAACCTCTCCCTGGATGTC-3 | (NM_001007079.1) |
IFNγ | forward 5′--GCCCTTCCTGTAACCAGATG-3 reverse 5′--ACACGACAGCCAAGTCAACG-3 | (NM_205149.1) |
TLR4 | forward 5′--TTTCCAAGCACCAGATAGCAACA-3 reverse 5′--TTCCAGCACAAGCCCTGAAATTA-3 | (NM_001030693.1) |