Table 1 List of antibodies used for immunohistochemistry and western blot detection
From: Lipid droplet dynamics in healthy and pyometra-affected canine endometrium
Antibody name | Method | Antibody typ | Source | Catalogue nr. | Dilution |
|---|---|---|---|---|---|
anti-PLIN1 (anti-PLIN1 rabbit polyclonal antibody) | IHC | primary | Abcam | ab3526 | 1:10000 |
anti-PLIN2 (ADRP (Perilipin 2) (AA5–27) mouse monoclonal antibody) | IHC | primary | AntibodiesOnline | ABIN112185 | 1:1000 |
anti-PLIN3 (TIP47 (F-10) mouse monoclonal antibody) | IHC | primary | Santa Cruz | sc-390,968 | 1:5000 |
anti-Ki-67 (Ki67 antigen clone MIB1 mouse monoclonal antibody) | IHC | primary | Agilent Technologies | M7240 | 1:1000 |
BrightVision, 1 step detection system goat anti-mouse HRP | IHC | secondary | ImmunoLogic | DPVM110HRP | RTU |
BrightVision, 1 step detection system goat anti-rabbit HRP | IHC | secondary | ImmunoLogic | DPVR110HRP | RTU |
anti-PLIN2 (ADRP/Perilipin 2 rabbit polyclonal antibody) | WB | primary | Proteintech | 15,294–1-AP | 1:500 |
anti-PLIN3 (TIP47 (F-10) mouse monoclonal antibody) | WB | primary | Santa Cruz | sc-390,968 | 1:500 |
anti-GAPDH (GAPDH [GT239] mouse monoclonal antibody) | WB | primary | GeneTex | GTX627408 | 1:5000 |
anti-α-tubulin (anti-α-tubulin rabbit polyclonal antibody) | WB | primary | Abcam | ab4074 | 1:1000 |
Amersham ECL rabbit IgG, HRP-linked whole AB (donkey) | WB | secondary | GE Healthcare | NA934 | 1:5000 |
Amersham ECL mouse IgG, HRP-linked whole Ab (sheep) | WB | secondary | GE Healthcare | NA931 | 1:5000 |