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Table 1 Detection rates of Rickettsia spp. from different tick species collected in Gangwon and Jeju provinces

From: Utility of ultra-rapid real-time PCR for detection and prevalence of Rickettsia spp. in ticks

Province

Tick species

Life stage

Number of ticks

Number of positive pools (MIR)

ITS

17 kDa

ompA

Gangwon

Haemaphysalis longicornis

Larva

2764

0

0

0

Nymph

50

1 (20.00)

1 (20.00)

1 (20.00)

Male adults

38

2 (52.63)

2 (52.63)

2 (52.63)

Female adults

240

2 (8.33)

1 (4.17)

2 (8.33)

Haemaphysalis flava

Larva

0

0

0

0

Nymph

93

0

0

0

Male adults

28

0

0

0

Female adults

10

0

0

0

Ixodes nipponensis

Larva

0

0

0

0

Nymph

16

0

0

0

Male adults

3

0

0

0

Female adults

30

0

0

0

Subtotal

3272

5 (1.53)

4 (1.22)

5 (1.53)

Jeju

Haemaphysalis longicornis

Larva

1470

0

0

0

Nymph

158

0

1 (6.33)

1 (6.33)

Male adults

99

1 (10.10)

1 (10.10)

1 (10.10)

Female adults

208

1 (4.81)

4 (19.23)

4 (19.23)

Haemaphysalis flava

Larva

0

0

0

0

Nymph

368

0

0

0

Male adults

30

0

0

0

Female adults

39

0

0

0

Subtotal

2372

2 (0.84)

6 (2.53)

6 (2.53)

Total

5644

7 (1.24)

10 (1.77)

11 (1.95)

  1. MIR Minimum infection rate depicted in ‰, ITS Internal transcribed spacer. ITS region of Rickettsia was detected by UR-qPCR, and other two genes (17 kDa protein antigen and ompA) were detected by conventional nested PCR