Fig. 2
From: Utility of ultra-rapid real-time PCR for detection and prevalence of Rickettsia spp. in ticks
Specificity of detection of Rickettsia species using ultra-rapid real-time PCR (UR-qPCR). The specificity of Rickettsia UR-qPCR is demonstrated by different melting temperatures observed when amplifying Rickettsia japonica recombinant DNA, Rickettsia sp. DNA from total nucleic acids isolated from tick sample, and the DNA of other common tick-borne pathogens, namely Anaplasma phagocytophilum, Ehrlichia chaffeensis, E. canis, Toxoplasma gondii, Borrelia burgdorferi, Coxiella burnetii, and samples with no DNA template (A). The melting temperature ranging from 76.03 °C to 77.01 °C were seen from detection PCR using DNA template of five different Rickettsia species (B). “N1” and “N2” are negative result using total nucleic acids isolated from two tick pools, and “N” is negative control without DNA template