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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Utility of ultra-rapid real-time PCR for detection and prevalence of Rickettsia spp. in ticks

Fig. 1

Sensitivity of detection of Rickettsia species using ultra-rapid real-time PCR (UR-qPCR). Amplification curves of UR-qPCR using 2.72 × 108 to 2.72 × 100 copies of Rickettsia DNA (denoted by number 8 to 0; A). The melting curves show Rickettsia detection is possible from 2.72 × 108 to 2.72 × 101 copies of target DNA (number 8–1; B). “N” is the negative control without a DNA template. Linear regression representing the relationship between cycle threshold of amplification (Ct value) and initial DNA copy number (C) was established by amplifying 10-fold dilutions of Rickettsia DNA from 2.72 × 108 to 2.72 × 101 DNA copies in triplicate

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