Fig. 1

TRIM4 inhibits PRRSV replication. A and B TRIM4 overexpression inhibited PRRSV replication. FLAG-TRIM4 (0.5 μg) or p3XFLAG-CMV-7.1 vector (0.5 μg) was transfected into Marc-145 cells at over 70% confluence grown in a 24-well plate for 24 h, and PRRSV at an MOI of 1.0 was inoculated into the cells for different time points (12, 24, 36, 48, 60, and 72 h); the virus titer was determined by means of TCID₅₀ (A), and the mRNA expression level of the PRRSV genome copy number was estimated by qRT-PCR (B). C–E The si-TRIM4 promotes PRRSV replication. The si-TRIM4 (60 nM) or 60 nM si-NC (60 nM) was transfected into Marc-145 cells at over 70% confluence grown in a 24-well plate for 24 h, and the TRIM4 protein level was quantified by western blotting (C). The si-TRIM4 (60 nM) or 60 nM si-NC (60 nM) was transfected into Marc-145 cells at over 70% confluence grown in a 24-well plate for 24 h, and PRRSV at an MOI of 1.0 was inoculated into the cells for different time points (12, 24, 36, 48, 60, and 72 h). The virus titer was characterized by TCID₅₀ (D). The difference in the mRNA expression of PRRSV genome copy number was investigated by qRT-PCR (E). *P < 0.05, **P < 0.01