Fig. 4

FAdV-4 induced the expression of inflammatory factors and activated the JAK2/STAT3 pathway in the broiler liver. After infection with FAdV-4 24 h (A), the virus load of FAdV-4 was determined by PCR with the primers of F1 gene, and the β-actin was used as a conference gene. The mRNA levels of (B) IL-6, (C) IL-1β, and (D) IFN-α, as well as the phosphorylation levels of (E) JAK2 and (F) STAT3, were determined by real-time qPCR and Western blot in the liver of broilers. (G) The degree of protein expression was determined by the depth of WB bands. Histogram is the result of the ratio of the phosphorylated protein to the total protein, and is the digital embodiment of the protein phosphorylation level of JAK2 and STAT3. Significance between the treatments was determined by T-test analysis using SPSS software (Version 20.0). Means with different alphabets (a, b) denotes significance at p < 0.05