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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Correction: Arginine regulates inflammation response-induced by Fowl Adenovirus serotype 4 via JAK2/STAT3 pathway

Fig. 2

Arginine treatment down-regulated inflammatory response and JAK2/STAT3 pathway-activated by FAdV-4 in LMH cells. The cells were divided into three groups: mock and FAdV-4 groups were cultured with a basal medium containing 10% FBS; ARG + FAdV-4 group was cultured with 300 μg/mL arginine medium. In addition, the FAdV-4 and the ARG + FAdV-4 groups were treated with the FAdV-4 strain NP for 2 h. On the contrary, the mock group was still cultured with a basic medium. After infection with FAdV-4 24 h (A), the virus load of FAdV-4 was determined by PCR with the primers of F1 gene, and the β-actin was used as a conference gene. The hepatic mRNA levels of (B) IL-6, (C) IL-1β, and (D) IFN-α were assessed. Similarly, the effect of arginine on the JAK2/STAT3 signaling pathway was determined by evaluating whether arginine interferes with the phosphorylated expression of JAK2 and STAT3 (E-F). (G) The degree of protein expression was determined by the depth of WB bands. Histogram is the result of the ratio of the phosphorylated protein to the total protein, and is the digital embodiment of the protein phosphorylation level of JAK2 and STAT3. Significance between the treatments was determined by T-test analysis using SPSS software (Version 20.0). Means with different alphabets (a, b) denotes significance at p < 0.05

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