Table 1 Geographic, animal and tissue origin of the examined samples, and Brucella identification based on various molecular techniques
Ν | Sample ID | Farm animal species | Originating tissue | Collection site | Screening PCR | MLVAa | BP26 | OMP31 | DNA quality (26/280 ratio) |
|---|---|---|---|---|---|---|---|---|---|
1 | 1751 | Billy goat | Testicle | Xanthi | B. melitensis REV1 | B. melitensis REV1 (2/8) | B. melitensis | – | < 1.2 |
2 | 1761 | Billy goat | Testicle | Xanthi | B. melitensis REV1 | B. melitensis REV1 (8/8) | B. melitensis | – | 1.8 – 2.2 |
3 | 1771 | Billy goat | Testicle | Xanthi | B. melitensis | B. melitensis (3/8) | B. melitensisB. | – | < 1.2 |
4 | 3111 | Billy goat | Testicle | Xanthi | B. melitensis REV1 | B. melitensis REV1 (8/8) | B. melitensis | – | 1.8 – 2.2 |
5 | 3131 | Ram | Testicle | Xanthi | B. melitensis | B. melitensis (8/8) | B. melitensis | – | 1.8 – 2.2 |
6 | 3141 | Billy goat | Testicle | Xanthi | B. melitensis | B. melitensis (8/8) | B. melitensis | – | 1.8 – 2.2 |
7 | 3171 | Ram | Testicle | Xanthi | B. melitensis REV1 | B. melitensis REV1 (8/8) | B. melitensis | – | 1.8 – 2.2 |
8 | 3142 | Billy goat | Spleen | Thessaloniki | B. melitensis | B. melitensis (2/8) | B. melitensis | – | < 1.2 |
9 | L81 | Bull | Lymph nodes | Thessaloniki | B. abortus | B. abortus (2/8) | B. abortus | – | < 1.2 |
10 | L84 | Bull | Lymph nodes | Thessaloniki | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
11 | L85 | Bull | Lymph nodes | Thessaloniki | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
12 | L111 | Bull | Lymph nodes | Thessaloniki | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
13 | L112 | Bull | Lymph nodes | Thessaloniki | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
14 | 51A | Cow | Embryonic rumen | Thiva | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
15 | 853 | Cow | Embryonic liver | Kalavryta | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
16 | 3201 | Cow | Embryonic rumen | Pella | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
17 | L11 | Cow | Embryonic rumen | Xanthi | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
18 | L12 | Cow | Embryonic liver | Xanthi | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
19 | L21 | Goat | Embryonic rumen | Thiva | B. melitensis | B. melitensis (8/8) | B. melitensis | – | 1.8 – 2.2 |
20 | L22 | Goat | Embryonic liver | Thiva | B. melitensis | B. melitensis (3/8) | B. melitensis | – | < 1.2 |
21 | L31 | Cow | Embryonic liver | Kozani | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
22 | L32 | Cow | Cotyledonary placenta | Kozani | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
23 | L61 | Cow | Embryonic rumen | Xanthi | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
24 | L62 | Cow | Embryonic liver | Xanthi | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
25 | L72 | Ewe | Embryonic liver | Lesvos island | B. melitensis | B. melitensis (3/8) | B. melitensis | – | < 1.2 |
26 | L93 | Ewe | Embryonic liver | Volos | B. melitensis | B. melitensis (8/8) | B. melitensis | – | 1.8 – 2.2 |
27 | L121 | Cow | Embryonic rumen | Xanthi | B. abortus | B. abortus (2/8) | B. abortus | – | < 1.2 |
28 | L122 | Cow | Embryonic liver | Xanthi | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
29 | L13 | Ewe | Cotyledonary placenta | Larisa | B. melitensis | B. melitensis (8/8) | B. melitensis | – | < 1.2 |
30 | L141 | Ewe | Embryonic rumen | Larisa | B. melitensis | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
31 | L201 | Ewe | Embryonic rumen | Fthiotida | B. melitensis REV1 | B. melitensis REV1 (8/8) | B. melitensis | – | 1.8 – 2.2 |
32 | L202 | Ewe | Embryonic liver | Fthiotida | B. melitensis | B. melitensis (3/8) | B. melitensis | – | < 1.2 |
33 | L221 | Cow | Embryonic rumen | Kozani | B. abortus | B. abortus (3/8) | B. abortus | – | < 1.2 |
34 | L222 | Cow | Embryonic liver | Kozani | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
35 | L223 | Cow | Cotyledonary placenta | Kozani | B. abortus | B. abortus (3/8) | B. abortus | – | < 1.2 |
36 | L231 | Goat | Embryonic rumen | Magnesia | B. melitensis | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
37 | L301 | Cow | Embryonic rumen | Trikala | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |
38 | L312 | Cow | Cotyledonary placenta | Florina | B. abortus | B. abortus (3/8) | B. abortus | – | < 1.2 |
39 | L317 | Cow | Embryonic liver | Florina | B. abortus | B. abortus (8/8) | B. abortus | – | 1.8 – 2.2 |