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Table 1 Geographic, animal and tissue origin of the examined samples, and Brucella identification based on various molecular techniques

From: Brucella spp. distribution, hosting ruminants from Greece, applying various molecular identification techniques

Ν Sample ID Farm animal species Originating tissue Collection site Screening PCR MLVAa BP26 OMP31 DNA quality (26/280 ratio)
1 1751 Billy goat Testicle Xanthi B. melitensis REV1 B. melitensis REV1 (2/8) B. melitensis < 1.2
2 1761 Billy goat Testicle Xanthi B. melitensis REV1 B. melitensis REV1 (8/8) B. melitensis 1.8 – 2.2
3 1771 Billy goat Testicle Xanthi B. melitensis B. melitensis (3/8) B. melitensisB. < 1.2
4 3111 Billy goat Testicle Xanthi B. melitensis REV1 B. melitensis REV1 (8/8) B. melitensis 1.8 – 2.2
5 3131 Ram Testicle Xanthi B. melitensis B. melitensis (8/8) B. melitensis 1.8 – 2.2
6 3141 Billy goat Testicle Xanthi B. melitensis B. melitensis (8/8) B. melitensis 1.8 – 2.2
7 3171 Ram Testicle Xanthi B. melitensis REV1 B. melitensis REV1 (8/8) B. melitensis 1.8 – 2.2
8 3142 Billy goat Spleen Thessaloniki B. melitensis B. melitensis (2/8) B. melitensis < 1.2
9 L81 Bull Lymph nodes Thessaloniki B. abortus B. abortus (2/8) B. abortus < 1.2
10 L84 Bull Lymph nodes Thessaloniki B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
11 L85 Bull Lymph nodes Thessaloniki B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
12 L111 Bull Lymph nodes Thessaloniki B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
13 L112 Bull Lymph nodes Thessaloniki B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
14 51A Cow Embryonic rumen Thiva B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
15 853 Cow Embryonic liver Kalavryta B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
16 3201 Cow Embryonic rumen Pella B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
17 L11 Cow Embryonic rumen Xanthi B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
18 L12 Cow Embryonic liver Xanthi B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
19 L21 Goat Embryonic rumen Thiva B. melitensis B. melitensis (8/8) B. melitensis 1.8 – 2.2
20 L22 Goat Embryonic liver Thiva B. melitensis B. melitensis (3/8) B. melitensis < 1.2
21 L31 Cow Embryonic liver Kozani B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
22 L32 Cow Cotyledonary placenta Kozani B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
23 L61 Cow Embryonic rumen Xanthi B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
24 L62 Cow Embryonic liver Xanthi B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
25 L72 Ewe Embryonic liver Lesvos island B. melitensis B. melitensis (3/8) B. melitensis < 1.2
26 L93 Ewe Embryonic liver Volos B. melitensis B. melitensis (8/8) B. melitensis 1.8 – 2.2
27 L121 Cow Embryonic rumen Xanthi B. abortus B. abortus (2/8) B. abortus < 1.2
28 L122 Cow Embryonic liver Xanthi B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
29 L13 Ewe Cotyledonary placenta Larisa B. melitensis B. melitensis (8/8) B. melitensis < 1.2
30 L141 Ewe Embryonic rumen Larisa B. melitensis B. abortus (8/8) B. abortus 1.8 – 2.2
31 L201 Ewe Embryonic rumen Fthiotida B. melitensis REV1 B. melitensis REV1 (8/8) B. melitensis 1.8 – 2.2
32 L202 Ewe Embryonic liver Fthiotida B. melitensis B. melitensis (3/8) B. melitensis < 1.2
33 L221 Cow Embryonic rumen Kozani B. abortus B. abortus (3/8) B. abortus < 1.2
34 L222 Cow Embryonic liver Kozani B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
35 L223 Cow Cotyledonary placenta Kozani B. abortus B. abortus (3/8) B. abortus < 1.2
36 L231 Goat Embryonic rumen Magnesia B. melitensis B. abortus (8/8) B. abortus 1.8 – 2.2
37 L301 Cow Embryonic rumen Trikala B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
38 L312 Cow Cotyledonary placenta Florina B. abortus B. abortus (3/8) B. abortus < 1.2
39 L317 Cow Embryonic liver Florina B. abortus B. abortus (8/8) B. abortus 1.8 – 2.2
  1. aParentheses indicate numbers of markers that worked properly in MLVA identification