Skip to main content

Table 1 Sequence of the oligonucleotides used for detection of resistance genes and phylotyping of E. coli isolates

From: Molecular characterization of blaNDM, blaOXA-48, mcr-1 and blaTEM-52 positive and concurrently carbapenem and colistin resistant and extended spectrum beta-lactamase producing Escherichia coli in chicken in Malaysia

Primers

Sequence (5’–3’)

Product size (bp)

Target gene

References

Pho-F

GTGACAAAAGCCCGGACACCATAAATGC

903

pho

[17]

Pho-R

TACACTGTCATTACGTTGCGGATTTGGCG

MCR1-F2

AGTCCGTTTGTTCTTGTGGC

320

mcr-1

[18]

MCR1-R2

AGATCCTTGGTCTCGGCTTG

MCR2-F

CAAGTGTGTTGGTCGCAGTT

715

mcr-2

MCR2-R

TCTAGCCCGACAAGCATACC

MCR3-F

AAATAAAAATTGTTCCGCTTATG

929

mcr-3

MCR3-R

AATGGAGATCCCCGTTTTT

MCR4-F

TCACTTTCATCACTGCGTTG

1116

mcr-4

MCR4-R

TTGGTCCATGACTACCAATG

MCR5-F

ATGCGGTTGTCTGCATTTATC

1644

mcr-5

MCR5-R

TCATTGTGGTTGTCCTTTTCTG

blaTEM-52 F

ATAAAATTCTTGAAGACGAAA

1080

blaTEM

[19]

blaTEM-52 R

GACAGTTACCAATGCTTAATC

blaCTX-F

CCCATGGTTAAAAAACACTGC

950

blaCTX

[20]

blaCTX-R

CAGCGCTTTTGCCGTCTAAG

NDM-F

GGTTTGGCGATCTGGTTTTC

621

blaNDM

[21]

NDM-R

CGGAATGGCTCATCACGATC

IMP-F

GGAATAGAGTGGCTTAAYTCTC

232

blaIMP

IMP-R

GGTTTAAYAAAACAACCACC

OXA-F

GCGTGGTTAAGGATGAACAC

438

blaOXA-48

OXA-R

CATCAAGTTCAACCCAACCG

ChuA.1

GAC GAA CCA ACG GTC AGG AT

279

chuA

[22]

ChuA.2

TGC CGC CAG TAC CAA AGA CA

YjaA.1

TGA AGT GTC AGG AGA CGC TG

211

yjaA

YjaA.2

ATG GAG AAT GCG TTC CTC AAC

TspE4.C2.1

TspE4.C2.2

GAG TAA TGT CGG GGC ATT CA

CGC GCC AAC AAA GTA TTA CG

152

TspE4.C2

AceK.f

AACGCTATTCGCCAGCTTGC

400

arpA

ArpA1.r

TCTCCCCATACCGTACGCTA

  

ArpAgpE.f

GATTCCATCTTGTCAAAATATGCC

301

arpA

ArpAgpE.r

GAAAAGAAAAAGAATTCCCAAGAG

  

trpAgpC.1

AGTTTTATGCCCAGTGCGAG

219

trpA

trpAgpC.2

TCTGCGCCGGTCACGCCC-

  

trpBA.f

CGGCGATAAAGACATCTTCAC

489

trpA

trpBA.r

GCAACGCGGCCTGGCGGAAG

 Â