Fig. 2

Characteristics of the CCEC-SV40T line. A PCR assays of SV40T mRNA. SV40T was detected in 293 T cells and different generations of CCEC-SV40T cells but not in CCECs. B Cellular morphology of CCEC-SV40T cells at 5, 10, 20, 30 and 40 generations. No morphological differences were observed. C Comparison of the proliferation ability between CCEC-SV40T cells and CCECs. The proliferation rate of CCEC-SV40T cells increased significantly compared with that of CCECs after 3 days (**P < 0.01). D Comparison of the cell cycle between CCECs and CCEC-SV40T cells. The percentage of cells in S phase was significantly higher for CCEC-SV40T than for CCEC-SV40T cells (**P < 0.01). E Immunofluorescence assays of different generations of CCEC-SV40T for cytokeratin 12. F Karyotype analysis of CCEC-SV40T cells and CCECs. G Serum dependence analysis of CCEC-SV40T cells and CCECs. Compared with 0%, 5%, and 10% serum concentrations, 20% serum concentrations significantly promoted cell proliferation (**P < 0.01 vs 0% group; #P < 0.05, ##P < 0.01 vs 20% group)