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Table 9 Parameters of gene-specific primers and reference genes obtained from the efficiency curve analysis in RT-qPCR

From: Broiler responses to copper levels and sources: growth, tissue mineral content, antioxidant status and mRNA expression of genes involved in lipid and protein metabolism

Tissue Gene T oC [cDNA] [Primer] Efficiency, % R2 Slope
Breast Muscle mTOR 60 45 ng/μl 200 mM 105 0.99 -3.201
GSK 3 60 45 ng/μl 200 mM 108 0.98 -3.135
AKT 1 60 45 ng/μl 400 mM 109 0.99 -3.131
CuZnSOD 60 45 ng/μl 200 mM 115 0.98 -3.013
MT 60 45 ng/μl 800 mM 109 0.99 -3.129
β-actin 60 45 ng/μl 200 mM 91.0 0.99 -3.548
Liver FAS 60 45 ng/μl 400 mM 94.0 0.98 -3.474
LPL 60 45 ng/μl 400 mM 108 0.99 -3.135
MDH 60 45 ng/μl 200 mM 104 0.99 -3.228
CuZnSOD 60 45 ng/μl 400 mM 103 0.99 -3.145
MT 60 45 ng/μl 400 mM 106 0.98 -3.192
β-actin 60 45 ng/μl 400 mM 109 0.98 -3.123
Intestine a CTR1 60 45 ng/μl 400 mM 96.0 0.99 -3.412
ATOX1 60 45 ng/μl 400 mM 101 0.99 -3.287
MT 60 45 ng/μl 400 mM 80.0 0.99 -3.917
STEAP1 60 45 ng/μl 400 mM 105 0.99 -3.199
GAPDH 60 45 ng/μl 400 mM 94.0 0.99 -3.482
  1. Abbreviations: mTOR Mechanistic target of rapamycin, GSK 3 Glycogen synthase kinase, AKT 1 Protein kinase serine-threonine 1, CuZnSOD Superoxide dismutase copper-zinc, MT Metallothionein, FAS Fatty Acid Synthetase, LPL Lipoprotein lipase, MDH Malate dehydrogenase, CTR1 Copper transporter 1, ATOX1 Antioxidant target 1 copper chaperone; STEAP1, Transmembrane Epithelial Antigene of the Prostate
  2. a Junction jejunum-ileum