Table 8 G. gallus genes, housekeeping genes for chickens and their specific primers used in RT-qPCR analysis
Genes | Description | Sequence 5 ' → 3' | Function |
|---|---|---|---|
FAS | Fatty Acid Synthetase | Fw: AGCGTGCTATGCTTGCC Rv: GTCCGTGACGAATTGCTTTAT | Lipid metabolism |
LPL | Lipoprotein lipase | Fw: TACAGTCTGGGTGCTCAT Rv: GCATACTCAAAGGTGGG | Lipid metabolism |
MDH | Malate dehydrogenase | Fw: AAAGACTGTGAAGGTGGTAG Rv: ATCCAAGCGAGTCAAGC | Lipid metabolism |
CuZnSOD | Superoxide dismutase copper-zinc | Fw: GGAGGAGTGGCAGAAGT Rv: TAAACGAGGTCCAGCAT | Antioxidant defense system |
MT | Metallothionein | Fw: AAGGGCTGTGTCTGCAAGGA Rv: CTTCATCGGTATGGAAGGTACAAA | Storage and transportation |
mTOR | Mechanistic target of rapamycin | Fw: GGCAATCAGTGTGCCTACCT Rv: GGCAGGCTCTTAAGGCAGAA | Protein Metabolism |
GSK 3B | Glycogen synthase kinase | Fw: GGGCTGTGTATTGGCTGAAC Rv: GTAGGCGTTCCCAGAACCTT | Protein Metabolism |
AKT 1 | Protein kinase serine-threonine 1 | Fw: ACGCTGACAGAAAACCGTGT Rv: ACAACTCCCCTCCGTTAGCA | Protein Metabolism |
SLC31A1 (CTR1) | Copper transporter 1 | Fw: CATCTTCAGGAGGTGGTCAT Rv: CAACGGCACATTCTCATAGC | Copper transporter |
ATOX1 | Antioxidant target 1 copper chaperone | Fw: AGATGTCGGAAAGGAGAGG Rv: AGAAGCAGCACCTCGAT | Copper Intracellular transporter |
STEAP1 | Transmembrane Epithelial Antigene of the Prostate 1 | Fw: CCTATTGTGGTCCTGCTCT Rev: AGCTTCCCAACCACATCT | Reduce Cu2+ to Cu1+ |
β-actin | Β-actin protein | Fw: GAGAAATTGTGCGTGACATCA Rv: CCTGAACCTCTCATTGCCA | Housekeeping gene |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | Fw: CAAGTGGACATTGTCGCCATCA Rv: GCTTCCCATTCTCAGCCTGACT | Housekeeping gene |