Broiler responses to copper levels and sources: growth, tissue mineral content, antioxidant status and mRNA expression of genes involved in lipid and protein metabolism

Table 7 Ingredient composition of the semi-purified basal diet (as fed basis)

^aP.A purism reagent exceeds standard ACS specification in trace metals analysis
^b0.03% L-lysine (79%); 0.27% L-arginine (98.5%); 0.40% L-glycine (98.5%); 0.85% L-alanine (99%); and 2.0% L-glutamic acid (100%). The amino acids alanine, glycine, and glutamic acid were added to maintain the ratio of essential nitrogen to total nitrogen at 0.50
^c0.055% coccidiostat; 0.010% avilamycin; 0.030% BHT; 1.02% sodium phytate; and 0.150% vitamin blend supplemented per kg of feed: vitamin A, 9750 IU; vitamin D₃, 2470 IU; vitamin E, 36.6 IU; vitamin B₁, 2.6 mg; vitamin B₂, 6.5 mg; vitamin B₆, 3.64 mg; vitamin B₁₂, 15 mcg; vitamin K, 1.95 mg; nicotinic acid, 39.0 mg; pantothenic acid, 13.0 g; folic acid, 0.91 mg; and biotin, 0.091 mg
^dMineral blend supplemented per kg of feed: 80 mg Fe; 10 mg Cu; 40 mg Zn; 0.3 mg Se and 1 mg I. Except for Cu added according each experimental treatment as 0; 4; 8; 12; and 16 mg Cu. Inorganic micromineral sources: Ferrous Sulphate (21.91% Fe); Zinc Sulphate (22.95% Zn); Copper Sulphate (25.13% Cu); Sodium Selenite (51.6% Se); Calcium Iodide (86% I) and Manganase Sulphate (30.3% Mn) and Organic micromineral sources: Bioplex®Fe (15% Fe); Bioplex®Zn (15.05% Zn); Bioplex®Cu (11.36% Cu); Selenium yeast (0.236% Se); Calcium Iodide (86% I); and Bioplex® Mn (13.86% Mn)
^eMicrobial phytase, 600 FTU/kg
^fAnalyzed value

ISSN: 1746-6148