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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Arginine regulates inflammation response-induced by Fowl Adenovirus serotype 4 via JAK2/STAT3 pathway

Fig. 5

Dietary 1.92% levels of arginine in broilers attenuated inflammatory response and inhibited JAK2/STAT3 signal transduction. Broilers were randomly separated into three experimental groups: Mock and FAdV-4 groups were given a standard diet; ARG + FAdV-4 group, given 1.92% of the arginine level standard diet. At 21 d, FAdV-4 and ARG + FAdV-4 groups were inoculated with FAdV-4, while the mock group was injected with normal saline. After infection with FAdV-4 24 h (A), the virus load of FAdV-4 was determined by PCR with the primers of F1 gene, and the β-actin was used as a conference gene. The mRNA levels of (B) IL-6, (C) IL-1β, and (D) IFN-α, as well as the phosphorylation levels of (E) JAK2 and (F) STAT3, were measured and analyzed. (G) The degree of protein expression was determined by the depth of WB bands. Histogram is the result of the ratio of the phosphorylated protein to the total protein, and is the digital embodiment of the protein phosphorylation level of JAK2 and STAT3. Significance between the treatments was determined by T-test analysis using SPSS software (Version 20.0). Means with different alphabets (a, b) denotes significance at p < 0.05

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