Fig. 1

FAdV-4 promoted inflammatory cytokines and the JAK2/STAT3 pathway in LMH cells. The inflammatory indexes of cells infected with or without FAdV-4 were evaluated. After infection with FAdV-4 24 h (A), the virus load of FAdV-4 was determined by PCR with the primers of F1 gene, and the β-actin was used as a conference gene. The mRNA levels of cytokines (B) IL-6, (C) IL-1β, and (D) IFN-α was determined by qPCR, while the phosphorylation of crucial proteins (p-JAK2 and p-STAT3) in the JAK2/STAT3 pathway was analyzed by WB (E-F). (G) The Western blotting was analyzed by gray scale and described by histogram, histogram is the result of the ratio of the phosphorylated protein to the total protein, and is the digital embodiment of the protein phosphorylation level of JAK2 and STAT3. Significance between the treatments was determined by T-test analysis using SPSS software (Version 20.0). Means with different alphabets (a, b) denotes significance at p < 0.05. β-actin was used as a control for protein loading, and the following were consistent. All values are expressed as Means ± SD (n = 6). The a, b, c, d bars in each panel without a common superscript letter were significantly different (P < 0.05). All remain consistent below unless otherwise stated