Fig. 2

Schematic diagram of the naturally recombinant FPV/MT270571 sequence. CPV-2c/KT156832 isolated in China and FPV/MK570646 from Australia served as the putative major and minor parents. A The potential recombination event was detected in the VP2 protein gene and was supported by similarity (2A) and bootscan (2B) analysis, which indicated that CPV-2c/KT156832 (red line) served as the main template of the complete VP2 gene, and the beginning of the VP2 gene was replaced by FPV/MK570646 (blue line). The FPV/MT270571 sequence served as the query. The y-axis indicated the percentage of nucleotide identity and permutated trees for the similarity plot and boot scanning, respectively, within a 200 bp-wide window with a 20-bp step size between plots. B The ML phylogenetic trees of the recombinant MT/270571 strains (♦) and it's major (▲) and minor (▼) putative parent strains over nucleotides 1–1,129 (2C) and 1,130–1,755 (2D). Bootstrap (1000 replications) values over 50% are shown for each node