Identification of extended-spectrum beta-lactamase (CTX-M)-producing Klebsiella pneumoniae belonging to ST37, ST290, and ST2640 in captive giant pandas

Su, Xiaoyan; Yan, Xia; Li, Yunli; Zhang, Dongsheng; Li, Lin; Geng, Yi; Su, Fei; Yue, Chanjuan; Hou, Rong; Liu, Songrui

doi:10.1186/s12917-022-03276-7

Table 1 Primers used for detection and sequencing of target genes in K. pneumoniae isolates

From: Identification of extended-spectrum beta-lactamase (CTX-M)-producing Klebsiella pneumoniae belonging to ST37, ST290, and ST2640 in captive giant pandas

Target	Forward primer (5'-3′)	Reverse primer (5′-3′)	Product size (bp)	Annealing temperature (°C)
β-Lactamases and ESBL-encoding genes
CTX-M	ATGTGCAGYACCAGTAARGT	TGGGTRAARTARGTSACCAGA	593	52
TEM	CATTTCCGTGTCGCCCTTATTC	CGTTCATCCATAGTTGCCTGAC	800	52
SHV	AGCCGCTTGAGCAAATTAAAC	ATCCCGCAGATAAATCACCAC	713	52
GES	AGTCGGCTAGACCGGAAAG	TTTGTCCGTGCTCAGGAT	399	52
PER	GCTCCGATAATGAAAGCGT	TTCGGCTTGACTCGGCTGA	520	52
VEB	CATTTCCCGATGCAAAGCGT	CGAAGTTTCTTTGGACTCTG	648	52
MGES gene
IS26	ATGAACCCATTCAAAGGCCGGCAT	TATGCAGCTTTGCTGTTACGACGG	387	55
intI1	CCGAGGATGCGAACCACTTC	CCGCCACTGCGCCGTTACCA	373	53
traA	CTGCTGTGGCGGCGTTCTTC	AGTAACCGGCGACCGACATACC	246	53
trbC	CGGYATWCCGSCSACRCTGCG	GCCACCTGYSBGCAGTCMCC	255	53
tnp513	CGAGTCAACCTCACACGCTTCC	TGCTCAATGACCTTCGGATCTTCG	269	55
tnpU	GCAAGGAGAAGCGACGAGTGTG	TACATGGCGGTCTCGGCTATCG	367	55
IS903	GCAATACGCACGCTTTCAGGC	ACTGCACGGTTACGGTCTGCA	521	55
Aminoglycoside genes
aac(3)-I	ACCTACTCCCAACATCAGCC	ATATAGATCTCACTACGCGC	169	55
aac(6’)-I	ATGAGTGGCTAAATCGATC	CCCGCTTTCTCGTAGCA	394	55
ant3-I	TGATTTGCTGGTTACGGTGAC	CGCTATGTTCTCTTGCTTTTG	284	55
aac(6')-Ib	TGACCTTGCGATGCTCTATG	TTAGGCATCACTGCGTGTT	497	53
rmtA	CTAGCGTCCATCCTTTCCTC	TTTGCTTCCATGCCCTTGCC	634	55
rmtB	CCCAAACAGACCGTAGAGGC	CTCAAACTCGGCGGGCAAGC	565	55
rmtD	CGGCACGCGATTGGGAAGC	CGGAAACGATGCGACGAT	750	55
armA	AGGTTGTTTCCATTTCTGAG	TCTCTTCCATTCCCTTCTCC	590	55
aadA5	ATGGGTGAATTYTTYCCTGCACAA	TCAACGCAAGATTCTCTCATTCGT	789	55
npmA	CGGGATCCAAGCACTTTCATACTGACG	CGGAATTCCAATTTTGTTCTTATTAGC	334	54
Quinolone’s genes
qnrA	AGAGGATTTCTCACGCCAGG	TGCCAGGCACAGATCTTGAC	578	53
qnrS	GCAAGTTCATTGAACAGGGT	TCTAAACCGTCGAGTTCGGCG	191	53
qnrB	GGMATHGAAATTCGCCACTG	TTTGCYGYYCGCCAGTCGAA	263	55
blaCTX-M group surrounding regions genes
ISEcpUP-F CTX-M-RCJ	CAAAATGATCCCCTCGTC	AGCGGCACACTTCCTAAC	1350–2700	55
IS26-FCJ CTX-M-RCJ	CATTTCAAAAACTCTGCTTAC	AGCGGCACACTTCCTAAC	800–1000	55

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ISSN: 1746-6148

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