Table 2 The primer used in qPCR
Name | Sequence (5’-3’) |
|---|---|
IL10-F | TTCAAACGAAGGACCAGATG |
IL10-R | CACAGGGCAGAAATTGATGA |
β-actin-F | GCGGGACATCAAGGAGAAG |
β-actin-R | AGGAAGGAGGGCTGGAAGAG |
gRNA-F | CCCTCCATGCCAAACTACCAC |
gRNA-R | TTGTCTTCTTTGGGTCCGTCT |
Leader-F | CACCTTGCTTCCGGAGTTG |
sgmRNA2-R | CAGCCAACCGGCGATTGTGAA |
sgmRNA3-R | GCAAAGCGGGCATACCGTGT |
sgmRNA4-R | ACGAAGTCTGATGCTGCGGTG |
sgmRNA5-R | CTGGCGTTGACGAGCACAGCA |
sgmRNA-6-R | CATCACTGGCGTGTAGGTAATGGA |
sgmRNA7-F | CCCGGGTTGAAAAGCCTCGTGT |
sgmRNA7-R | GGCTTCTCCGGGTTTTTCTTCCTA |