Table 1 The primer of constructing the plasmid construction and PRRSV infection clones
Name | Sequence (5’-3’) |
|---|---|
N-pca-F-EcoRI | CCGAATTCATGCCAAATAACAACGGCAAGC |
N-pca-R-Xhol | TTCTCGAGTCATGCTGAGGGTGATGCTG |
N-pca-R-122 | TTCTCGAGTCATGCCGGCGGTGATGCTG |
XmaI-F | CACGTCGAAAGTGCCGCG |
KpnI-122-R | GATGTGCTGCAAGGCGAT |
BbvCI -R | CCGCATGGTTCTCGCCAATTA |
N36A-F | CATCGCCCAACAAAACCAGGCCAGAGGCAA |
N36A-R | CCCCGGTCCCTTGCCTCTGGCCTGGTTTTG |
N70A-F | GGCATCACTTTACCCCTGCTGAGCGGCAAT |
N70A-R | CAGACACAATTGCCGCTCAGCAGGGGTAAA |
N77A-F | GTGAGCGGCAATTGTGTCTGGCGTCGATCC |
N77A-R | GAAGGCAGTCTGGATCGACGCCAGACACAA |
N78A-F | GGCAATTGTGTCTGTCGGCGATCCAGACTG |
N78A-R | CTGATTGAAGGCAGTCTGGATCGCCGACAG |
N93A-F | GCGCTGGAACTTGTGCCCTGGCAGATTCAG |
N93A-R | AACTTATCCTCCCTGAATCTGCCAGGGCAC |
N95A-F | GGAACTTGTGCCCTGTCAGATGCAGGGAGG |
N95A-R | AGTGTAACTTATCCTCCCTGCATCTGACAG |
N99A-F | GTCAGATTCAGGGAGGATAGCTTACACTGT |
N99A-R | AAACTAAACTCCACAGTGTAAGCTATCCTC |
N122A-F | CCGCGCCACAGCATCACCCGCAGCATGATG |
N122A-R | GAATGCCAGCCCATCATGCTGCGGGTGATG |