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Fig. 5 | BMC Veterinary Research

Fig. 5

From: A novel amino acid site of N protein could affect the PRRSV-2 replication by regulating the viral RNA transcription

Fig. 5

Growth characterization of the A78 in PAMs (A): The multistep kinetics of the mutated viruses in PAMs, the cells were infected 0.01 MOI PRRSV, the supernatants were collected at various time points and titrated. The viral titres were calculated by the Reed-Muench method; (B): The gRNA qPCR results of the A78 and XH-GD in PAMs. The viral RNA was extracted and subjected to qPCR analysis, and the change in gRNA levels compared with β-actin was determined. Each data point represents the mean value of triplicates. (*, P < 0.05; **, P < 0.01; ***, P < 0.001 in comparison with the XH-GD group)

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BMC Veterinary Research

ISSN: 1746-6148

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