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Fig. 4 | BMC Veterinary Research

Fig. 4

From: A novel amino acid site of N protein could affect the PRRSV-2 replication by regulating the viral RNA transcription

Fig. 4

Growth characterization of the mutated viruses. A: The multistep kinetics of the mutated viruses. Marc-145 cells were infected with PRRSV at an MOI of 0.1. The supernatants were collected at various time points and titrated. The viral titres were calculated by the Reed-Muench method; B: The gRNA qPCR results of the mutated viruses. Viral RNA was extracted and subjected to qPCR analysis, and the change in gRNA levels compared with β-actin was determined. Each data point represents the mean value of triplicates. (*, P < 0.05; **, P < 0.01; ***, P < 0.001 in comparison with the XH-GD group). ($: WT vs. A78; *: WT vs. A105)

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