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Fig. 1 | BMC Veterinary Research

Fig. 1

From: A novel amino acid site of N protein could affect the PRRSV-2 replication by regulating the viral RNA transcription

Fig. 1

Substituting amino acids 36, 78, 99 and 105 of the N protein from serine to alanine decreased the IL-10 mRNA level. The plasmids were transfected into PAM cells (3D4/2). After 48 h, the samples were collected for qPCR analysis. Compared with β-actin, the relative IL-10 mRNA level was determined. Each data point represents the mean value of triplicates (*, P < 0.05; **, P < 0.01; ***, P < 0.001 in comparison with the XH-GD group)

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