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Table 1 Sequences of primers and dual-labeled probes used in the assay

From: Molecular characterization of porcine reproductive and respiratory syndrome virus (PRRSv) identified from slaughtered pigs in northern Uganda

Genotype

Name

Orientation

Sequence

PRRSv-1

Primer 1

Forward

5′-CGA CCA CCT CAC CCA GAC-3′

Primer 1

Reverse

5′-CAG TTC CTG CGC CTT GAT-3′

Probe

Genomic

5′-6-FAM-CCT CTG CTT GCA ATC GAT CCA GAC-BHQ1–3’

PRRSv-2

Primer 2

Forward 1

5′-ATG ATG RGC TGG CAT TCT-3’

Primer 2

Reverse

5′-ACA CGG TCG CCC TAA TTG-3’

Probe

Genomic

5′-HEX-TGT GGT GAA TGG CAC TGA TTG ACA-BHQ2–3’