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Table 1 Minimum inhibitory concentrations for isolates recovered in this study

From: Evaluating the potential of third generation metagenomic sequencing for the detection of BRD pathogens and genetic determinants of antimicrobial resistance in chronically ill feedlot cattle

Sample

No

BRD

Pathogen

Culture Result

AMP

TIO

CLIN*

DANO

ENRO

FLOR

GAM

GEN*

NEO*

PEN

SPECT

SUL*

TET

TIAM*

TILD

TILM

TRIMS*

TULA

TYL*

CTET

OTET

1

M. bovis

 

-

-

-

-

0.12

4

128

-

-

16

-

-

-

-

256

512

-

64

256

4

2

2

M. bovis

 

-

-

-

-

0.12

4

64

-

-

16

-

-

-

-

256

512

-

8

32

8

4

3

P. multocida

Few

0.25

0.25

32

0.12

0.12

0.50

16

4

64

0.12

128

512

8.0

64

32

32

2

128

64

-

-

5

M. bovis

 

-

-

-

-

8.00

8

512

-

-

16

-

-

-

-

256

512

-

512

256

4

4

5

P. multocida

1 + 

0.25

0.25

32

0.12

0.12

0.50

1

4

8

0.12

32

256

0.5

32

1

4

2

8

32

-

-

6

P. multocida

2 + 

0.50

0.25

32

0.12

0.12

0.50

2

4

64

1.00

32

512

8.0

32

2

16

4

8

64

-

-

7

M. bovis

 

-

-

-

-

4.00

4

256

-

-

16

-

-

-

-

256

512

-

512

32

16

8

7

P. multocida

1 + 

0.25

0.25

16

0.12

0.12

0.50

1

1

4

0.25

32

512

0.5

8

1

2

2

8

16

-

-

8

M. bovis

 

-

-

-

-

4.00

8

512

-

-

16

-

-

-

-

256

512

-

512

256

8

4

8

P. multocida

1 + 

0.25

0.25

32

0.12

0.12

0.50

1

2

8

0.12

32

256

0.5

16

1

4

2

8

32

-

-

9

M. bovis

 

-

-

-

-

4.00

4

512

-

-

16

-

-

-

-

256

512

-

512

256

8

4

9

P. multocida

4 + 

0.50

0.25

32

0.12

0.12

0.50

2

4

64

2.00

32

512

4.0

64

2

16

4

8

64

-

-

10

M. bovis

 

-

-

-

-

0.12

2

32

-

-

16

-

-

-

-

128

256

-

8

32

8

4

10

P. multocida

1 + 

0.25

0.25

32

0.12

0.12

0.50

1

4

16

0.25

32

512

0.5

32

1

4

2

8

32

-

-

11

M. bovis

 

-

-

-

-

2.00

2

512

-

-

16

-

-

-

-

256

512

-

2

256

1

1

11

P. multocida

1 + 

0.25

0.25

32

0.12

0.12

0.50

1

4

8

0.12

32

256

0.5

32

1

8

2

8

64

-

-

12

M. bovis

 

-

-

-

-

4.00

2

512

-

-

16

-

-

-

-

256

128

-

512

256

4

2

12

P. multocida

3 + 

4.00

0.25

32

0.12

0.12

0.25

1

4

64

16.0

32

512

4.0

64

2

8

4

8

64

-

-

13

M. bovis

 

-

-

-

-

0.50

4

512

-

-

16

-

-

-

-

256

512

-

512

64

4

2

14

M. bovis

 

-

-

-

-

0.12

4

128

-

-

16

-

-

-

-

128

256

-

64

64

4

2

14

P. multocida

1 + 

16.0

0.25

32

0.12

0.12

0.50

2

4

64

16.0

32

512

4.0

64

2

16

4

8

64

-

-

17

M. haemolytica

1 + 

0.25

0.25

8

0.12

0.12

1.00

16

2

4

0.25

32

512

0.5

16

1

16

2

64

64

-

-

18

P. multocida

2 + 

0.25

0.25

32

0.12

0.12

0.25

1

2

64

0.50

32

512

4.0

32

2

8

4

8

64

-

-

19

P. multocida

4 + 

0.25

0.25

32

0.12

0.12

0.50

1

2

8

0.25

16

256

0.5

32

1

8

2

8

64

-

-

20

P. multocida

1 + 

4.00

0.25

32

0.12

0.12

0.50

2

4

64

8.0

32

512

8.0

64

2

16

4

8

64

-

-

23

P. multocida

1 + 

0.25

0.25

32

0.12

0.12

0.50

1

2

8

0.25

32

256

0.5

32

1

8

2

8

64

-

-

24

M. haemolytica

1 + 

0.25

0.25

8

0.12

0.12

0.50

1

1

4

0.12

32

256

0.5

16

1

8

2

8

64

-

-

24

P. multocida

1 + 

0.25

0.25

32

0.12

0.12

0.50

1

2

8

0.12

32

256

0.5

32

2

8

2

8

64

-

-

25

M. bovis

 

-

-

-

-

0.12

4

512

-

-

16

-

-

-

-

256

512

-

512

256

8

4

25

P. multocida

1 + 

0.50

0.25

32

0.12

0.12

0.50

1

2

8

0.12

32

256

0.5

32

1

8

2

8

64

-

-

  1. Samples were screened for the presence of BRD pathogens and recovered isolates were tested for sensitivity to antimicrobials via serial broth microdilution using the BOPO7F panel. MICs for each antimicrobial were compared against CLSI breakpoints (full details in Supplementary Table 2, Additional File 2. Table entries with a dash indicate an isolate was not tested against a certain antimicrobial. Antimicrobials without established breakpoints for the target organisms are indicated with an asterisk
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BMC Veterinary Research

ISSN: 1746-6148

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