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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Baseline T-lymphocyte and cytokine indices in sheep peripheral blood

Fig. 3

Detection of single-cell expression of IFN-γ and IL-4 by ELISpot. PBMCs were added to ELISpot plates in Con A (5 μg/ml), PMA (20 ng/ml) + Ionomycin (1 μg/ml), or medium in triplicate and cultured at 37 °C and 5% CO2 for 24 h. Spots were counted using an ELISpot reader system; mean numbers of spot-forming cells (SFCs) are shown per 105 PBMCs. A SFC of IFN-γ stimulated under different conditions. B SFC of IL-4 stimulated under different conditions. C ELISpot representative images of IFN-γ activated with Con A and PMA + Ionomycin. D ELISpot representative images of IL-4 activated with Con A and PMA + Ionomycin. Data are presented as means ± SD with statistically significant differences between stimulation groups (****p < 0.001)

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