Fig. 3From: Establishment of enzyme-linked immunosorbent assays based on recombinant S1 and its truncated proteins for detection of PEDV IgA antibodyIdentification of recombinant S1 truncated protein expression. AÂ SDS-PAGE of S1 and its truncated fragments with Coomassie blue staining; BÂ western blotting analysis of the S1 and its truncated fragments with anti-His McAb; CÂ western blotting analysis of S1T1 with anti-PEDV S1 McAb-6B4; DÂ western blotting analysis of S1T2 with anti-PEDV S1 McAb-6G1; EÂ western blotting analysis of S1T3 with anti-PEDV S1 McAb-1C11; FÂ western blotting analysis of S1 protein and its truncated fragments with PEDV positive pig serum. (Lane1: PEDV-S1; Lane2: PEDV-S1T1; Lane3: PEDV-S1T2; Lane4: PEDV-S1T3; Lane5: His tag positive control)Back to article page