Table 1 Cytoplasmic microinjection efficiency of transposon systems in bovine embryos
From: Application of transposon systems in the transgenesis of bovine somatic and germ cells
Concentration | IVM | Microinjection | IVC | |||
|---|---|---|---|---|---|---|
Higha | No. COCs | Condition | No. 8-cells (%) | Total Blastocysts (%) | GFP Expressing Blastocysts (%) | Total Cell Number |
122 | Wild type | 79 (64.8) | 41 (33.6) | 0 (0) | 92.64 ± 23.69 | |
130 | PiggyBac | 60 (46.2) | 17 (13.1) | 6 (35.3) | 89.20 ± 14.82 | |
130 | Sleeping Beauty | 51 (39.2) | 15 (11.5) | 10 (66.7) | 86.40 ± 23.52 | |
130 | Tol2 | 67 (51.5) | 23 (17.7) | 11 (47.8) | 96.00 ± 18.58 | |
Lowb | No. COCs | Condition | No. 8-cells (%) | Total Blastocysts (%) | GFP Expressing Blastocysts (%) | Total Cell Number |
78 | Wild type | 51 (65.38) | 21 (26.92) | 0 (0) | 100.00 ± 33.08 | |
77 | PiggyBac | 31 (40.26) | 14 (18.18) | 6 (42.86) | 95.50 ± 25.03 | |
77 | Sleeping Beauty | 30 (38.96) | 10 (12.99) | 7 (70.00) | 102.00 ± 28.58 | |
77 | Tol2 | 31 (40.26) | 15 (15.58) | 9 (75.00) | 100.78 ± 24.99 | |