Fig. 3

Ulvans promote MDV reactivation. (A) MDV transformed T-lymphocytes (3867 K) were treated with vitamin complex (VitComp), Searup® or purified ulvan extract at indicated concentrations or left untreated (NT). At 24, 48, 72 and 96 hpt, cells were stained with the Fixable Viability Dye eFluor™ 780. The percentage of cells in which MDV reactivates from latency (UL47-GFP positive cells) was then estimated by flow cytometry within the viable cell population (eFluor negative). Data of three independent experiments are represented as means +/− SEM. Statistics analyses did not show any difference (P > 0.05). (B) Representative cytometry dot plots obtained after 96 h of culture without treatment (NT) or with 2 ml/l of compounds. (C) The 3867 K cells were treated with VitComp, Searup® or ulvan extract (at 2 ml/l) in combination with an inducer of viral reactivation, the sodium butyrate (NaBut) used at 0.5 mM. Viral reactivation was estimated by flow cytometry within the viable cell population at 24, 48 and 72 hpt. Data of three independent experiments are represented as means +/− SEM. * p < 0.05; ** p < 0.005