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Table 3 Summary of the molecular analyses conducted on samples collected from L/157, B/113, and B/120

From: First glanders cases detected in Nepal underscore the need for surveillance and border controls

 

real-time PCR

  

PCR-HRM clustering

B. pseudomallei complex

B.mallei fliP

B. pseudomallei

 

Horse Id

Sample

IPC

aroA

fliP

orf11

 
  

(Cq value)

(Cq value)

(Cq value)

(Cq value)

 

L/157

swab

30.3

31.6

29.4

-

L2B2s B2 India – Group_2 (large)

B/113

swab

30

37.6

34.2

-

L2B2s B2 India – Group_2 (large)

B/115

swab

30

40a

-

-

/

B/117

swab

30.2

-

-

-

/

B/1

pus

32

35.5

33.3

-

L2B2s B2 India – Group_2 (large)

  1. IPC Internal control with exogen DNA, - (negative), / (not done), PCR-HRM High-resolution melting PCR analysis for the genotyping of Burkholderia mallei
  2. avalue beyond the cut-off (sample considered as negative)