First glanders cases detected in Nepal underscore the need for surveillance and border controls

BMC Veterinary Research

Table 2 Serological results from the five equids tested with the complement fixation test, ELISA and Luminex^® methods

				ID Screen ® Glanders indirect ELISA^a		GLANDA ELISA^b		Luminex – Hcp1		Luminex - GroEL		Glyco Elisa^c
		CFT		Cut-off > 50 (40<S/P >= 50 : undet.)		cut-off > 50		cut-off > 43		cut-off > 45		cut-off > 40
Animal ID	species	Titer	Result	%S/P	Result	%S/P	Result	%S/P	Result	%S/P	Result	%S/P	Result
L/157	Horse	4441	P	119	P	281	P	121	P	123	P	150	P
B/113	Mule	444443	P	117	P	301	P	100	P	110	P	164	P
B/115	Mule	0	n	80	P	285	P	62	P	90	P	9	n
B/117	Horse	0	n	47	U	288	P	146	P	45	U	2	n
B/120	Horse	444444	P	129	P	288	P	145	P	147	P	193	P

^aELISA test based on a semi-purified fraction of B. mallei, ^bELISA test based on a recombinant protein of B. mallei, ^cELISA test based on a glycoengineered antigen of Burkholderia
P Positive, U Undetermined, n Negative
For CFT, result were expressed as the intensity of hemolysis inhibition (0=0%, 1=25%, 2=50%, 3=75%, and 4=100%) for reach serum dilution (1/5, 1/10, 1/20, 1/40 and 1/80)
For ELISA and Luminex methods, results were expressed as S/P =((Sample – Negative control)/(Positive control - Negative control))^* 100. Cut-off values for each test are mentioned

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