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Fig. 6 | BMC Veterinary Research

Fig. 6

From: NADH oxidase of Mycoplasma hyopneumoniae functions as a potential mediator of virulence

Fig. 6

A rNOX, rGroEL, and PBS were applied to examine fibronectin activity with indirect ELISA. *** represents an extremely significant difference (p < 0.001), while “ns” represents no significant difference between groups (p >0.05). B “a,” SDS–PAGE analysis of purified recombinant protein rNOX and BSA. Lane M is pre-stained protein mass markers; Lane 1: purified recombinant protein rNOX; Lane 2: BSA. The gel in this figure was cropped and the full-length gel was presented in Supplementary FigureS3. “b,” Mhp NOX with fibronectin interaction analysis by far Western blot. Lane rNOX: PVDF membrane with transferred Mhp NOX protein incubated with fibronectin and the anti-fibronectin antibody. Lane BSA: PVDF membrane with transferred BSA (negative control) incubated with fibronectin, and the anti-fibronectin antibody. The blots in this figure were cropped and the full-length blots were presented in Supplementary FigureS4.

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