Fig. 5

rNOX adhesive inhibition assays with real-time PCR and CCU₅₀ assay. A Adhesion rates were calculated by real-time PCR for Mhp bacterial counting. Adhesion rate = (number of Mhp antigens collected from infected cells incubated with the anti-rNOX serum/ number of Mhp antigens collected from infected cells in the group incubated with the pre-immune sera) × 100. Quantitative real-time PCR analysis was performed and expressed as log10 DNA copy number per mL of Mhp strain-infected cell distribution against P97. Data are presented as the mean ± SD of three independent experiments (***: p<0.001). B Mhp titers were quantified using a CCU₅₀ assay. Data are presented as the mean ± SD of three independent experiments (***: p<0.001; **: p<0.01).