Table 1 Antibody responses and viral titers in the swabs, bloods, and tissues of Anatidae intranasally inoculated with clade 2.3.4.4 H5N6 HPAIV and observed for 10Â days
Species | Inoculum titer (log EID50) | ID | Sexa | Ageb | Serum antibody | Viral titer (log EID50/mL) | Viral titer (log EID50/g) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
cELISA | HI titer | Pharyngolaryngeal / cloacal swabs | Conjunctival swab | Blood | Tissues | |||||||||||||
Pre | Pre | Post | 0d | 1d | 2d | 3d | 5d | 7d | 10d | Pancreas | Other tissuesc | |||||||
Eurasian wigeon | 6 | W104 | M | J |  +  |  < 2 | 32 |  − d/ −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  −  |  −  |
W105 | M | A |  −  |  < 2 | 32 |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  −  |  −  | ||
W106 | M | J |  −  |  < 2 | 32 |  − / −  |  ≤ 1.0 / −  |  ≤ 0.6 / −  | 4.3 / 2.5 | 2.7 / 2.5 |  − / −  |  − / −  |  −  |  −  |  ≤ 1.8 |  −  | ||
4 | W107 | M | A |  +  | 2 | 4 |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  −  |  −  | |
W108 | M | J |  −  |  < 2 | 128 |  − / −  | 4.5 / −  | 1.2 / −  | 4.0 / 3.5 | 4.5 / 2.5 |  − / 2.5 |  − / ≤ 1.2 |  −  |  −  |  −  |  −  | ||
2 | W109 | M | A |  +  | 4 | 16 |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  −  |  −  | |
W110 | M | J |  −  |  < 2 |  < 2 |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  −  |  −  | ||
Mallard | 6 | M114 | M | J |  −  |  < 2 | 64 |  − / −  | 3.5 / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  ≤ 1.4 |  −  |
M115 | F | J |  +  |  < 2 | 64 |  − / −  |  ≤ 1.3 / −  | 1.5 / −  | 6.3 / −  | 2.5 / −  | 3.3 / −  |  ≤ 0.4 / −  |  −  |  −  |  −  |  −  | ||
M116 | M | A |  −  |  < 2 | 16 |  − / −  | 2.3 / −  | 1.7 / ≤ 1.3 | 4.5 / −  |  ≤ 1.0 / −  |  − / −  |  − / −  | 2.5 |  −  |  ≤ 1.4 |  −  | ||
4 | M117 | F | J |  −  |  < 2 | 256 |  − / −  | 4.2 / −  | 3.7 / −  | 3.7 / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  −  |  −  | |
M118 | M | A |  +  | 2 | 4 |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  −  |  −  | ||
2 | M119 | F | J |  −  |  < 2 |  < 2 |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  −  |  −  | |
M120 | M | J |  +  |  < 2 |  < 2 |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  −  |  −  | ||
Northern pintail | 6 | P124 | F | A |  −  |  < 2 | 128 |  − / −  | 2.5 / −  | 1.7 / −  |  − / −  | 3.7 / −  |  ≤ 0.6 / −  |  − / −  |  −  |  −  |  ≤ 2.0 |  −  |
P125 | M | A |  −  |  < 2 | 128 |  − / −  | 2.7 / −  | 3.5 / −  | 5.5 / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  −  |  −  | ||
P126 | F | J |  −  |  < 2 | 64 |  − / −  | 2.2 / −  |  ≤ 0.7 / −  |  ≤ 0.7 / −  |  ≤ 3.6 / −  |  − / −  |  − / −  |  −  |  −  |  −  |  −  | ||
4 | P127 | F | J |  −  |  < 2 |  < 2 |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  −  |  −  | |
P128 | F | A |  −  |  < 2 |  < 2 |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  −  |  −  | ||
2 | P129 | F | A |  −  |  < 2 |  < 2 |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  ≤ 1.6 |  −  | |
P130 | F | J |  −  |  < 2 |  < 2 |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  − / −  |  −  |  −  |  ≤ 1.3 |  −  | ||