Table 1 The primer sequences of Real-time PCR
Gene | Primer | Primer sequence | Size of PCR product |
|---|---|---|---|
REV | F | TTGTTGAAGGCAAGCATCAG | 105Â bp |
R | GAGGATAGCATCTGCCCTTT | ||
ALV-J | F | TGCGTGCGTGGTTATTATTTC | 144Â bp |
R | AATGGTGAGGTCGCTGACTGT | ||
ITGα1 | F | CCAGTAGGAAGAGACAGCCAAT | 161 bp |
R | TAAGCATAGAGCGGTCCACAT | ||
ITGα3 | F | CTCAACCTCACGCTGCTGGA | 121 bp |
R | GCACTTCTGACTTCGCCTTCTT | ||
ITGα5 | F | GTACATCTACAGCGGGAGGG | 132 bp |
R | TTGCCATCCAGGTCGGTGT | ||
ITGα6 | F | GGTTCCTGTCAGCAAGGTGTT | 186 bp |
R | CTTATCTTGGCGGCTCTCATCA | ||
ITGα8 | F | GTGGAAGGAGGAGCGGTGTA | 110 bp |
R | GGTTCTCTGGTGCCATTGACTT | ||
ITGα9 | F | GCAGGCTTCTTCACCGAGGA | 195 bp |
R | ATCCGTGGTAGTTGGCTGAGAG | ||
ITGα11 | F | CTTCGTCTGCTTCACTGCCATC | 132 bp |
R | TGCCGCTCACCACTCTCATC | ||
ITGβ3 | F | ACTTCTCCTGTGTCCGCTTCAA | 101 bp |
R | GCAGTAGTCACCAGTCCAGTCT | ||
GADPH | F | GAACATCATCCCAGCGTCCA | 132Â bp |
R | CGGCAGGTCAGGTCAACAAC |