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Fig. 4 | BMC Veterinary Research

Fig. 4

From: TMT-based proteomic analysis reveals integrins involved in the synergistic infection of reticuloendotheliosis virus and avian leukosis virus subgroup J

Fig. 4

Integrins are associated with the synergistic infection of REV and ALV-J. A The qRT–PCR results of eight Integrins, including ITGα1, ITGα3, ITGα5, ITGα6, ITGα8, ITGα9, ITGα11 and ITGβ3 in CEFs, were consistent with the TMT-based proteomic analysis. The data represent the mean ± SEM determined from three independent experiments (n = 3), with each experiment containing three technical replicates. B ALV-J and REV synergistically enhanced the ITGα5 and ITGβ3 protein levels, and declined ITGα1 and ITGα9 protein levels in CEFs at 72 hpi as detected by western blot with anti-ITGα5 antibody, anti-ITGβ3 antibody, anti- ITGα1 antibody and anti-ITGα9 antibody. Western blot images obtained subsequent to running a Simple Western™ System (ProteinSimple)

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