Fig. 5From: Molecular identification and functional characterization of IRF4 from common carp (Cyprinus carpio. L) in immune response: a negative regulator in the IFN and NF-κB signalling pathwaysExpression analysis of ccIRF4 in response to A. hydrophila challenge in vivo. Total RNA was extracted from the spleen (A), head kidney (B), foregut (C) and hindgut (D) at 0 (as control), 3, 6, 12, 24, 48 and 72 h post injection for real-time PCR. Expression was normalized using the 40S ribosomal protein S11 (n = 3, mean ± SD, *P < 0.05)Back to article page