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Table 1 Mix Ratio to qPCR [14]

From: Glutathione S-transferase (GST) and cortisol levels vs. microbiology of the digestive system of sheep during lambing

Component

Volume in a 10 μl reaction

Primer (F + R)

1 μl (0.8 μM)

DNA matrix

2 μl (0.04–0.015 × 10ˉ4)

SsoAdvanced™ Universal SYBR® Green Supermix

5 μl

Sterile water

2 μl

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BMC Veterinary Research

ISSN: 1746-6148

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