Fig. 2

Histologic image from post-mortem examination of case #1. A Pulmonary parenchyma and pleura are infiltrated and expanded by inflammatory nodules consisting of macrophages, multinucleated giant cells, lymphocytes, and plasma cells. The dashed line delineates the transition between healthy pulmonary parenchyma and a pulmonary lesion at the top of the image. Hematoxylin and eosin (H&E) stain. 4 × magnification. Scale bar represents 300 microns. B Intralesional macrophages (arrow) contain basophilic, rod-shaped bacteria. H&E stain. 40 × magnification. C Macrophages (insert) laden with acid-fast staining bacteria. Fite's acid-fast stain. 40 × magnification. Histology sections were examined using a conventional light microscope (Olympus BX51) equipped with a digital camera (Olympus DP26) and cellSens Standard image analysis software (Olympus, Center Valley, PA 18034, USA). Images were acquired at 300 dpi resolution and were edited for scale bar and white balance in Photoshop 23.1.1 (Adobe, San Jose, CA 95110, USA)