Detection of porcine parainfluenza virus type-1 antibody in swine serum using whole-virus ELISA, indirect fluorescence antibody and virus neutralizing assays

Table 7 Study C: PPIV-1 vaccine study. Percent of anti-PPIV-1 antibody positive pigs at vaccination or exposure (0 DPV) and 11, 18, 24, 31, 38, 43, and 48 DPV. A total of 50 piglets were included in this study. Treatment groups included live exposure/challenged (LE/C), non-vaccinated/challenged (NV/C), non-vaccinated/non-challenged (NV/NC), RNA particle vaccinated/challenged (RP/C), and adjuvanted RNA particle/challenged (RPAdj/C). Assay thresholds were determined by ROC analysis at a specificity of 99% while maximizing sensitivity. Values within an experimental group by DPV with different superscripts were significantly different at p ≤ 0.05 by McNemar’s test. Asterisks indicate significant Cochran’s Q test by DPV but nonsignificant individual McNemar’s pairwise testing

^ASamples with a sample/positive (S/P) ratio greater than 0.14 were considered positive. Samples with a S/P ratio less than 0.14 were considered negative
^BSamples with an IFA reciprocal titer greater than or equal to 40 were considered positive. Samples with an IFA titer less than 40 were considered negative
^CSamples with a VN titer greater than or equal to 20 were considered positive. Samples with a VN titer less than 20 were considered negative
¹Antibody positive/treatment group size; wv-ELISA Whole virus ELISA, IFA Indirect fluorescence antibody, SVN Serum virus neutralization

ISSN: 1746-6148