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Table 2 Subtyping of C. felis isolates using reference positions based on gp60 gene

From: Molecular prevalence and subtyping of Cryptosporidium spp. in fecal samples collected from stray cats in İzmir, Turkey

Sample

R1

(463–556)

Deletion

(667–679)

Indel

(706–711)

R2

(770–910)

Indel

(1015–1068)

Indel

(1156–1167)

Subtype family

C. felis cat isolate 1

1 copy

9 bp

-

2 copy

-

4 GGT

XIXa

C. felis cat isolate 2

1 copy

9 bp

-

1 copy

-

a

XIXa

C. felis cat isolate 3

1 copy

9 bp

-

2 copy

-

a

XIXa

C. felis cat isolate 4

1 copy

9 bp

-

2 copy

-

a

XIXa

C. felis cat isolate 5

1 copy

9 bp

-

2 copy

-

3 GGT

XIXa

C. felis cat isolate 6

1 copy

9 bp

-

1 copy

-

4 GGT

XIXa

C. felis cat isolate 7

1 copy

9 bp

-

1 copy

-

4 GGT

XIXa

C. felis cat isolate 8

1 copy

9 bp

-

1 copy

-

2 GGT

XIXa

C. felis cat isolate 9

1 copy

9 bp

-

2 copy

-

3 GGT

XIXa

C. felis cat isolate 10

1 copy

9 bp

-

2 copy

-

2 GGT

XIXa

C. felis cat isolate 11

1 copy

9 bp

-

2 copy

-

4 GGT

XIXa

C. felis cat isolate 12

1 copy

9 bp

-

1 copy

-

4 GGT

XIXa

Reference isolate 1

1–3 copies

3 or 9 bp

-

1–4 copies

-

2–5 GGT

XIXa

Reference isolate 2

1 or 2 copies

6 or 15 bp

-

2–4 copies

18 bp

4 GGT

XIXb

Reference isolate 3

2 copies

15 bp

-

3 copies

-

3 GGT

XIXc

Reference isolate 4

1 copy

-

-

1 copy

-

4 GGT

XIXd

Reference isolate 5

1 copy

-

6 bp

1 copy

36 bp

4 GGT

XIXe

  1. Reference positions are from Jiang et al. 2020 [26]
  2. ashows the unevaluated sequences because of sequencing errors
  3. R1: 33-bp tandem repeat (CCACCTAGTGGCGGTAGTGGCGTGTCCCCTGCT)
  4. R2: 39-bp tandem repeat (AGCACAACTACGGCTACAGCGAGCACTGCGAGTTCGACA)