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Table 4 Motility parameters of the canine post-thaw sperm after cryopreservation in extenders containing different levels of melatonin versus the controls

From: Supplementation of melatonin to cooling and freezing extenders improves canine spermatozoa quality measures

 

Group

Negative Control

Positive Control

M1

M2

M3

TM

49.52 ± 5.69 b

35.82 ± 7.65 ab

25.57 ± 3.21 a

54.64 ± 8.24 b

50.45 ± 6.90 b

PM

21.41 ± 3.06 d

13.07 ± 2.58 bc

6.76 ± .77 ab

20.9 ± 2.35 d

18.90 ± 1.78 cd

VCL

20.03 ± 2.68 c

13.70 ± 3.26 ac

8.35 ± 1.00 a

18.27 ± 3.12 bc

19.76 ± 2.25 bc

VAP

11.00 ± 1.24 c

7.08 ± 1.61 ac

4.32 ± .55 a

10.50 ± 2.17 bc

10.14 ± .97 bc

VSL

7.65 ± .92 c

4.57 ± 1.00 ac

2.65 ± .35 a

6.67 ± 1.31 bc

6.50 ± .45 bc

ALH

1.30 ± .19 ab

1.05 ± .22 ab

.73 ± .09 a

1.47 ± .31 b

1.38 ± .20 b

BCF

2.23 ± .39 ab

1.47 ± .43 ab

.75 ± .10 a

2.31 ± .60 b

2.39 ± .33 b

MAD

23.72 ± 3.59 ab

15.70 ± 4.16 ab

8.99 ± 1.07 a

24.93 ± 6.68 b

23.26 ± 2.65 b

STR

69.48 ± 2.23 b

64.68 ± 1.24 a

61.05 ± .70 a

64.04 ± 1.10 a

64.62 ± 1.83 a

LIN

38.61 ± 2.11 b

33.63 ± 1.25 a

31.52 ± .60 a

36.25 ± 2.08 ab

33.43 ± 1.61 a

WOB

55.47 ± 1.65

51.94 ± .98

51.62 ± .52

56.65 ± 3.41

51.65 ± 1.12

  1. A one-way ANOVA statistical test was used to make a comparison between treatment groups. LSD posthoc test was used to perform pairwise comparisons. Negative Control (without Melatonin or DMSO), Positive Control (containing DMSO), M1 (containing 0.5 mM Melatonin), M2 (containing 1 mM Melatonin), and M3 (containing 2 mM Melatonin). different letters in the superscripts indicate a statistically significant difference (P ≤ 0.05) between study groups. All numbers are presented as Means±SEM. VCL Velocity Curvilinear (μm/s), VAP Velocity Average Path (μm/s), VSL Velocity Straight Path (μm/s), ALH Amplitude of Lateral Head Displacement (μm), BCF Beat Cross Frequency (Hz), MAD Mean Angular Displacement (°), STR Straightness (%), LIN Linearity (%), and WOB Wobble (%)